Effects of functional training on the performance of sedentary college students / Efeitos do treinamento funcional sobre o desempenho de universitários sedentários / Efectos del entrenamiento funcional en el rendimiento de universitarios sedentarios

ABSTRACT Introduction: One focus of current research by frontline physical education teachers has always been the elective teaching of college students who do not play sports. Lack of training time and poor student training make many professional badminton teaching methods impossible for elective courses. Objective: Study the impact of functional training on the badminton performance of college students. Methods: Forty-four sedentary college students were randomly selected and equally divided into the experimental and control group. The functional training strategy was used in the experimental group, while the control group used the traditional badminton training method. The experimental and control groups were trained twice weekly for 60 minutes and 12 weeks. The data obtained were compared, analyzed, and discussed statistically. Results: In terms of the FMS test, the total score of the experimental group was optimized by 5.61, and the total score of the control group was optimized by 3.20. Regarding badminton performance, the experimental group showed an increase of 23.12 points, while the control group increased 16.75 points (P<0.01), indicating a very significant difference in the results. Conclusion: This work evidenced the importance of promoting functional training, which is superior to the current sports teaching methods. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: Um dos focos das pesquisas atuais dos professores de educação física na linha de frente tem sido sempre o ensino eletivo de estudantes universitários que não praticam esportes. A falta de tempo de treinamento e a deficiente formação dos estudantes tornam muitos métodos profissionais de ensino de badminton impossíveis de serem realizados em cursos optativos. Objetivo: Estudar o impacto do treinamento funcional sobre o desempenho de badminton dos estudantes universitários. Métodos: Foram selecionados aleatoriamente 44 universitários sedentários, divididos igualmente em grupo experimental e controle. No grupo experimental foi utilizada a estratégia de treinamento funcional, enquanto o grupo de controle usou o método tradicional de treinamento de badminton. Tanto o grupo experimental quanto o grupo controle treinaram duas vezes por semana, por 60 minutos e durante 12 semanas. Os dados obtidos comparados, analisados e discutidos estatisticamente. Resultados: Em termos de teste FMS, a pontuação total do grupo experimental foi otimizada em 5,61, e a pontuação total do grupo de controle foi otimizada em 3,20. Com relação ao desempenho do badminton, o grupo experimental apresentou um aumento de 23,12 pontos, enquanto que o grupo controle aumentou 16.75 pontos (P<0.01), indicando que houve uma diferença muito significativa dos resultados. Conclusão: Este trabalho evidenciou a importância da promoção do treinamento funcional, comparativamente superior aos métodos de ensino esportivo praticados atualmente. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: Uno de los focos de la investigación actual de los profesores de educación física de primera línea ha sido siempre la enseñanza optativa de los estudiantes universitarios que no practican deportes. La falta de tiempo de entrenamiento y la escasa formación de los alumnos hacen que muchos métodos profesionales de enseñanza del bádminton resulten imposibles para los cursos optativos. Objetivo: Estudiar el impacto del entrenamiento funcional en el rendimiento en bádminton de estudiantes universitarios. Métodos: Se seleccionaron aleatoriamente 44 estudiantes universitarios sedentarios y se dividieron equitativamente en grupo experimental y grupo de control. En el grupo experimental se utilizó la estrategia de entrenamiento funcional, mientras que el grupo de control utilizó el método tradicional de entrenamiento de bádminton. Tanto el grupo experimental como el de control entrenaron dos veces por semana, durante 60 minutos y durante 12 semanas. Los datos obtenidos se comparan, analizan y discuten estadísticamente. Resultados: En términos de la prueba FMS, la puntuación total del grupo experimental se optimizó en 5,61, y la puntuación total del grupo de control se optimizó en 3,20. En cuanto al rendimiento en bádminton, el grupo experimental mostró un aumento de 23,12 puntos, mientras que el grupo de control aumentó 16,75 puntos (P<0,01), lo que indica que hubo una diferencia muy significativa en los resultados. Conclusión: Este trabajo evidenció la importancia de promover el entrenamiento funcional, comparativamente superior a los métodos de enseñanza deportiva practicados actualmente. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Strength and quality training of basketball players / Força e treinamento de qualidade dos jogadores de basquetebol / Fuerza y entrenamiento de calidad de los jugadores de baloncesto

ABSTRACT Introduction: Quality exercise training of young people is a fundamental basic requirement for developing physical fitness in college basketball teaching. Objective: Enhance exercise training in young basketball players. Methods: A random selection of 50 student volunteers was made for this research. The strength and quality of basketball players of different ages were analyzed. The experimental group was subjected to specific strength training, while the control group was subjected to routine physical exercises. The experiment period was 12 weeks, and the specific technical indicators of the athletes were collected before and after the end of the experiment. The collected data were statistically treated and discussed in light of the scientific literature. Results: No significant difference was found between the two groups in the initial test score (P>0.05). The experimental group's performance in exercise was better than the control group after 12 weeks (P<0.01). The experimental group demonstrated better performance than the control group in the standing jump, 15-meter reentry, and forward jump, corroborating the significantly different data between the two groups (P<0.01). Conclusion: This study aimed to study the unique role of strength training in the physical training of young basketball players. Special strength training positively affects upper limb muscle training in young basketball players. The lower extremities of basketball players showed expressive functional gains with special strength training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O exercício físico de qualidade dos jovens é requisito básico fundamental para o desenvolvimento da aptidão física no ensino do basquetebol universitário. Objetivo: Fortalecer o treinamento do exercício físico em jovens jogadores de basquetebol. Métodos: Efetuou-se a seleção aleatória de 50 estudantes voluntários para a pesquisa. Foi analisada a força e a qualidade dos jogadores de basquetebol em diferentes idades. O grupo experimental foi submetido a um treinamento de força específica, enquanto o grupo de controle foi submetido a exercícios físicos de rotina. O período do experimento foi de 12 semanas e os indicadores técnicos específicos dos atletas foram coletados antes e após a finalização do experimento. Os dados coletados foram tratados estatisticamente e discutidos à luz da literatura científica. Resultados: Não foi encontrada diferença significativa entre os dois grupos na pontuação inicial do teste (P>0,05). O desempenho do grupo experimental no exercício foi melhor que o do grupo de controle após as 12 semanas (P<0,01). O grupo experimental demonstrou um desempenho melhor que o do grupo controle no salto em pé, reentrada de 15 metros, e salto de frente, corroborando com os dados significativamente distintos entre os dois grupos (P<0,01). Conclusão: Este estudo teve como objetivo estudar o papel único do treinamento de força no treinamento físico dos jovens jogadores de basquetebol. O treinamento especial de força afeta positivamente o treinamento muscular dos membros superiores nos jovens jogadores de basquetebol. As extremidades inferiores dos jogadores de basquetebol evidenciaram ganhos funcionais expressivos com o treinamento especial de força. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El ejercicio físico de calidad de los jóvenes es un requisito básico fundamental para el desarrollo de la aptitud física en la enseñanza del baloncesto universitario. Objetivo: Reforzar el entrenamiento del ejercicio físico en jóvenes jugadores de baloncesto. Métodos: Se realizó una selección aleatoria de 50 estudiantes voluntarios para la investigación. Se analizó la fuerza y la calidad de los jugadores de baloncesto a diferentes edades. El grupo experimental fue sometido a un entrenamiento de fuerza específico, mientras que el grupo de control fue sometido a ejercicios físicos rutinarios. El periodo del experimento fue de 12 semanas y se recogieron los indicadores técnicos específicos de los atletas antes y después de finalizar el experimento. Los datos recogidos fueron tratados estadísticamente y discutidos a la luz de la literatura científica. Resultados: No se encontraron diferencias significativas entre los dos grupos en la puntuación de la prueba inicial (P>0,05). El rendimiento del grupo experimental en el ejercicio fue mejor que el del grupo de control después de 12 semanas (P<0,01). El grupo experimental mostró un mejor rendimiento que el grupo de control en el salto de pie, el reingreso de 15 metros y el salto frontal, corroborando los datos significativamente diferentes entre los dos grupos (P<0,01). Conclusión: Este estudio tenía como objetivo estudiar el papel único del entrenamiento de la fuerza en la preparación física de los jóvenes jugadores de baloncesto. El entrenamiento de fuerza especial afecta positivamente al entrenamiento muscular de las extremidades superiores en jóvenes jugadores de baloncesto. Las extremidades inferiores de los jugadores de baloncesto mostraron ganancias funcionales expresivas con el entrenamiento de fuerza especial. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Repetitive transcranial magnetic stimulation for treatment of limb spasticity following multiple sclerosis: a systematic review and meta-analysis

Pilot trials have suggested that repetitive transcranial magnetic stimulation (rTMS) may reduce limb spasticity in multiple sclerosis (MS). We carried out the current meta-analysis to synthesize currently available evidence regarding such correlation. Up to November 2022, five international electronic databases (Cochrane CENTRAL, PubMed, Embase, Web of Science, and CINAHL) and four Chinese electronic databases (CBM, CNKI, WanFang Data, and VIP) were systematically searched to identify randomized trials comparing active rTMS and sham stimulation in patients with MS-related spasticity. Two reviewers independently selected studies and extracted data on study design, quality, clinical outcomes, and time points measured. The primary outcome was clinical spasticity relief after intervention. Secondary outcomes included spasticity at the follow-up visit 2 weeks later and post-treatment fatigue. Of 831 titles found, we included 8 studies (181 participants) in the quantitative analysis. Pooled analyses showed that rTMS therapy was associated with significant spasticity relief in the early post-intervention period [standardized mean differences (SMD): -0.67; 95%CI: -1.12 to -0.21], but there was insufficient evidence for rTMS in reducing spasticity at the follow-up visit 2 weeks later (SMD: -0.17; 95%CI: -0.52 to 0.17) and fatigue (SMD: -0.26; 95%CI: -0.84 to 0.31). This evidence supports the recommendations to treat MS-related spasticity with rTMS, but underlines the need for further large randomized trials.

Study on HPV vaccine hesitation and associated factors among female college students in Weifang / 中国学校卫生

Objective@#To investigate HPV vaccine hesitation and associated factors among female college students in Weifang, and to provide a suggestions for improving the coverage rate of HPV vaccine among female college students.@*Methods@#The questionnaire of HPV vaccine hesitancy of female college students was designed. By adopting the stratified sampling method, the survey was conducted among female students at one medical university and three nonmedical universities in Weifang. Chi-square test and Logistic regression were used to analyze the influencing factors of vaccine hesitation.@*Results@#Female college students who had high risk perception toward vaccine (OR=4.15, 95%CI=1.55-11.10) and those who were angry about the sideeffects of the vaccine (OR=3.63, 95%CI=1.95-6.75) were hesitate to vaccinate against HPV. Female college students who believed long protection period of HPV vaccine (OR=0.08, 95%CI=0.04-0.17), that women had a high probability of cervical cancer (OR=0.44, 95%CI=0.21-0.90) and that the knowledge of HPV vaccine scored >6 (OR=0.31, 95%CI=0.15-0.67) were more likely to receive HPV vaccine.@*Conclusion@#High risk perception of vaccines and anger at vaccine adverse reactions are important factors affecting HPV vaccines for female college students. Our findings call for strategic role of the state, school-based health education as well as medical professional guidance in lowering HPV vaccination hesitation among fenale college sutdents.

Agreement of self-reported and proposed standard on oral health among children / 中国学校卫生

Objective@#To analyze the consistency between self-reported oral health and proposed standard in evaluating children oral health status, and to test whether self-reported oral health can be used in children population.@*Methods@#The clinical examination data and questionnaire data were 2 158 children aged 12-year-old in Beijing, China. Child oral health status was measured by self-reported oral health and proposed standard from World Health Organization (WHO), and was classified into "good, general, poor" based on each method. Kappa test was used to evaluate the consistency of two measurements. Using multivariate nonordinal logistic regression to identify the influencing factors of self-reported oral health, and to explore the possible reasons for the consistency differences of two measurements.@*Results@#According to the WHO standard, 25.5%, 65.3% and 9.2% children oral health were rated as good, general and poor, respectively. The proportion was 50.6%, 37.7% and 11.8% respectively based on self-report method. Kappa value for two measurements was 0.04, indicating poor agreement between two methods. Results from regression model show that both the untreated caries (OR=2.47, P<0.01) and toothache (OR=3.54, P<0.01) were associated with self-reported oral health, while not observed in periodontal health (OR=1.34, P=0.18) and oral hygiene status(OR=0.95, P=0.75).@*Conclusion@#Self-reported oral health results can reflect caries status to some extents among children, but it failed to reflect other aspects, such as periodontal health status and oral hygiene. Self-reported oral health among children should be considered based on the requirements of assessment content and accuracy in the future work.

Biochanin A attenuates myocardial ischemia/reperfusion injury through the TLR4/NF-κB/NLRP3 signaling pathway

Abstract Purpose: Myocardial ischemia/reperfusion (Ml/R) injury is a leading cause of damage in cardiac tissues, with high rates of mortality and disability. Biochanin A (BCA) is a main constituent of Trifolium pratense L. This study was intended to explore the effect of BCA on Ml/R injury and explore the potential mechanism. Methods: In vivo MI/R injury was established by transient coronary ligation in Sprague-Dawley rats. Triphenyltetrazolium chloride staining (TTC) was used to measure myocardial infarct size. ELISA assay was employed to evaluate the levels of myocardial enzyme and inflammatory cytokines. Western blot assay was conducted to detect related protein levels in myocardial tissues. Results: BCA significantly ameliorated myocardial infarction area, reduced the release of myocardial enzyme levels including aspartate transaminase (AST), creatine kinase (CK-MB) and lactic dehydrogenase (LDH). It also decreased the production of inflammatory cytokines (IL-1β, IL-18, IL-6 and TNF-α) in serum of Ml/R rats. Further mechanism studies demonstrated that BCA inhibited inflammatory reaction through blocking TLR4/NF-kB/NLRP3 signaling pathway. Conclusion: The present study is the first evidence demonstrating that BCA attenuated Ml/R injury through suppressing TLR4/NF-kB/NLRP3 signaling pathway-mediated anti-inflammation pathway.

Cyclo-oxygenase-2 promotes migration and invasion of breast cancer MDA-MB231 cells by regulating EMT / 中国肿瘤生物治疗杂志

@#Objective:To investigate the role of cyclo-oxygenase-2 (COX-2) in breast cancer metastasis and its possible mechanism. Methods: A total of 45 cases of primary breast cancer tissues and brain metastatic breast cancer tissues were collected from patients, who underwent mastectomy in Yunnan Cancer Hospital from October 2015 to April 2018, including 30 cases of primary lesions and 15 cases of brain metastasis. qPCR was used to detect the expression of COX-2 in breast cancer tissues and brain metastatic breast cancer tissues. Recombinant viruses with COX-2 over-expression (LV6-COX2) or COX-2 knockdown (LV3-COX2 shRNA1, LV3-COX2 shRNA2) were transfected into human breast cancer MDA-MB-231 cells; After obtaining the stable expression cell lines, the effect of COX-2 expression on the proliferation of MDA-MB-231 cells was detected by CCK-8, and the effects of COX-2 expression on the migration and invasion of MDA-MB-231 cells were detected by scratch test and Transwell assay, respectively. The mRNAand protein expressions of COX-2 in each group were examined by qPCR and WB, respectively. The effect of COX-2 expression on the expression of EMT-related genes in MDA-MB-231 cells was analyzed by qPCR. Results: The expression of COX-2 in tissues of patients with brain metastases was significantly higher than that in patients with primary breast cancer tissues (P<0.01), and it was correlated with tumor TMN stage in breast cancer patients. MDA-MB-231 cell lines with stable COX-2 over-expression/knockout were successfully constructed. Over-expression of COX-2 promoted the migration and invasion of MDA-MB-231 cells (all P<0.01), and significantly increased the expressions of MMP2, MMP1, N-cadherin and vimentin (all P<0.01), but exerted insignificant effect on cell proliferation. The effect of COX-2 silence exerted the opposite effect and promoted cell proliferation (P<0.05). Conclusion: COX-2 is highly expressed in brain metastatic breast cancer tissues, which may promote the migration and invasion of breast cancer MDA-MB-231 cells by regulating EMT processes.

Effect of myeloid-derived suppressor cells on B cell function in mice bearing breast cancer / 中国肿瘤生物治疗杂志

@# Objective: To investigate the effect of myeloid-derived suppressor cells (MDSCs) from mice bearing breast cancer on the function of normal B cells. Methods:ABABL/c mouse 4T1 breast cancer model was established. The spleen MDSCs of tumor-bearing mouse and normal mouse spleen B cells were sorted by magnetic beads, and the sorted MDSCs and B cells were co-incubated. Flow cytometry was used to test the effect of MDSCs on the expressions of B cell surface molecules, including PD-1, PD-L1, CTLA-4, CCR6, CD62L and MHCⅡ; ELISA assay was used to detect the secretion of IgA, IgM and IgG by B cells; BrdU kit was used to detect B cell proliferation; andAnnexin V/PI staining was used to detect B cell apoptosis. B cells in the co-culture system were again sorted by magnetic beads and were then co-cultured with T cells; BrdU kit was used to detect T cell proliferation, and Annexin V/PI was used to detect T cell apoptosis. Results: Compared with B cell control group, the expression of PD-L1 on B cells in B+MDSC group was increased (P<0.01), while the expressions of PD-1, CTLA-4, CCR6, CD62L and MHC Ⅱ were all decreased (all P<0.01); The IgA, IgM and IgG secreted by B cells were significantly increased (all P<0.01); the proliferation of B cells was increased (P<0.01) and the apoptosis was decreased (P<0.01). Compared with the T cell control group, the proliferation of T cells in the B+MDSC (1:5) +T group was significantly reduced (P<0.01); however, there was no significant difference in T cell apoptosis. Conclusion: MDSCs from breast cancer bearing mice promotes B cell proliferation and inhibits B cell apoptosis, and the MDSC-induced B cells can inhibit T cell proliferation.

Determination of Gallic Acid,Quercetin and Kaempferol in Gongyanping Capsules by HPLC / 中国药师

Objective:To establish an HPLC method for the determination of gallic acid,quercetin and kaempferol in Gongyanping capsules. Methods:HPLC was applied with the chromatographic conditions as follows: The chromatographic column was Agilent-SB C18 (250 mm × 4. 6 mm,5 μm) at 30℃; the mobile phrase was methanol-0. 3% phosphoric acid solution with gradient elution; the flow rate was 1. 0 ml·min-1. Results: The linearity relationship of gallic acid, quercetin and kaempferol was within the range of 98.200-491.00 μg·ml-1(r=0.999 9), 7.520-37.600 μg·ml-1(r=0.999 9) and 4.940-24.700 μg·ml-1(r=0.999 9), re-spectively;the average recovery was 96. 74%(RSD=1. 33%), 98. 18%(RSD=1. 70%) and 97. 04%(RSD=1. 28%),respective-ly. Conclusion:The method is simple, accurate and repeatable.

Research progress of overactivation mechanism of sympathetic nervous system in obesity-related hyper-tension / 心血管康复医学杂志

Obesity is an independent risk factor for hypertension.Overactivation of sympathetic nervous system caused by obesity is an important path inducing hypertension.This article made following overview on research pro-gress of overactivation mechanism of sympathetic nervous system in obesity-related hypertension.

A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates

Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter, Aeromonas, Enterobacter, Leclercia, Microvirga, Pseudomonas, Rhizobium, Planococcus, Paenisporosarcina and Planomicrobium. In addition, a total of 14 species belonging to 8 genera in 3 phyla (Proteobacteria, Firmicutes, Actinobacteria) were isolated, Curtobacterium, Massilia, Pseudomonas and Sphingomonas were the dominant genera. Although some of the genera isolated were inconsistent with those detected by molecular method, both of two methods proved that many different endophytic bacteria coexist in G. montana. According to the potential functional analyses of these bacteria, some species are known to have possible beneficial effects on hosts, but whether this is the case in G. montana needs to be confirmed.

In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10-10M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed significant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the first report that documents the effect of cigarette smoke components on oocyte maturation and sperm capacitation. The present findings demonstrate the adverse effects of smoke constituents of mammalian reproduction and the differences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.

Prevalence and risk factors of peripheral arterial diseases in male Chinese octogenarians and nonagenarians with hypertension / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the prevalence and risk factors of peripheral arterial disease (PAD) in male Chinese octogenarians and nonagenarians with hypertension.</p><p><b>METHODS</b>Ankle-brachial index (ABI) was measured in the noninvasive vascular laboratory for hypertensive male octogenarians and nonagenarians enrolled from outpatient and inpatient departments. The baseline conditions were investigated using standard questionnaire and by routine physical examinations. PAD was diagnosed when an ABI≤0.9 in either lower extremity.</p><p><b>RESULTS</b>Totally 290 male Chinese octogenarians and nonagenarians [age: (84.61±4.20) years] with hypertension who were receiving antihypertensive therapy were enrolled in this study, among whom 9 men with missing data except age and ABI measurements. The ABI was 0.948±0.258, with the range of highest frequency of 0.91-1.30, and 106 patients were diagnosed as PAD, 182 as non-PAD, and 2 had ABI>1.3. ABI in hypertensive men with PAD were significantly lower than in those without PAD (P<0.05). On the contrary, age, blood urea nitrogen, white blood cell counts, platelets and aspartic transaminase were significantly higher in PAD patients than in non-PAD patients (all P<0.05). The prevalence of PAD in this study population were 36.5%; more specifically, it significantly differed between different subgroups when stratified by age (28.6% vs. 46.3%, below and above 85 years), with and without hypertension (27.5% vs. 40.1%), stroke (44.7% vs. 27.5%), dyslipidemia (41.4% vs. 33.3%), coronary artery disease (44.1% vs. 13.9%), and diabetes mellitus (53.7% vs. 21.8%) (all P<0.05). The prevalences of PAD in hypertensive patients treated with diuretics, calcium antagonists, beta-blocker, or angiotensin receptor antagonist were 41.4%, 36.1%, 22.4%, and 26.8%, respectively. No association was observed between the prevalence of PAD and smoking/alcohol drinking in these subjects. Multivariate analysis showed that age (OR 1.12, 95%CI 1.014-1.238), blood urea nitrogen (OR 1.15, 95%CI 1.025-1.301), aspartic transaminase (OR 1.05, 95%CI 1.005-1.089), diabetes mellitus (OR 4.02, 95%CI 1.797-9.009), coronary artery disease (OR 6.34, 95%CI 1.734-23.214) were strong risk factors of PAD.</p><p><b>CONCLUSION</b>PAD is highly prevalent among aged Chinese hypertensive men, in which age, blood urea nitrogen, aspartic transaminase, diabetes mellitus, coronary artery disease may be involved in the development of this condition.</p>

Interleukin-10 Is Expressed in HepG2.2.15 Cells and Regulated by STAT1 Pathway / 华中科技大学学报（医学）（英德文版）

This study investigated the expression profiles of IL-10 gene in three human hepatoma cell lines including Huh7,HepG2,and HepG2 transfected with a plasmid containing hepatitis B virus (HBV) named HepG2.2.15.RT-PCR analysis demonstrated that IL-10 message RNA was absent in HepG2 and Huh7 cells,whereas it was present in HepG2.2.15 cells,which was consistent with ELISA result.Furthermore,except for lamivudine other antiviral treatments did not significantly decrease the HBV DNA level in HepG2.2.15 cells,while they had different effects on the expression of IL-10 protein,although stimulation by LPS had no significant effect.In addition,except for poly(I:C),the other treatments decreased the expression of IL-10 protein to different degrees,but had no significant effects on the expression of NF-κB and MyD88.Meanwhile,all treatments we used had effect on the expression of STAT1.In conclusion,IL-10 was expressed in HepG2.2.15 cells and STAT1 pathway might be involved in the regulation of IL-10 expression in HepG2.2.15 cells,but it was not the sole pathway,the exact mechanism warrants further study.

Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations

The MYF5 gene is first inducibly expressed in muscle cell during embryonic muscle development and plays an important role in regulating the differentiation of skeletal muscle precursors. In this study we used PCR-RFLP to investigate two pig (Sus scrofa) populations (n = 302) for two MYF5 gene polymorphisms, a previously unreported novel Met-Leu shift single nucleotide polymorphism (SNP) MYF5/Hsp92II located on exon 1 and the previously identified intron 1 MYF5/HinfI SNP. Haplotype and association analysis showed that haplotypes of the two SNPs were significantly associated with drip loss rate (DLR, p < 0.05), water holding capacity (WHC, p < 0.05), biceps femoris meat color value (MCV2, p < 0.05), biceps femoris marbling score (MM2, p < 0.01), longissimus dorsi intramuscular fat percentage (IMF, p < 0.01) and longissimus dorsi Water moisture content (WM, p < 0.01) in the population 2. However, further studies are needed to confirm these preliminary results.

Analysis of bcl-6 protein expression and gene rearrangement in diffuse large B-cell lymphoma / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate bcl-6 protein expression and gene rearrangement patterns in diffuse large B-cell lymphoma (DLBCL) and their clinicopathologic significance.</p><p><b>METHODS</b>Immunohistochemical studies for bcl-6 and CD10 proteins were performed on 51 cases of DLBCL paraffin-embedded tissues (including 22 nodal samples and 29 extranodal samples) and 10 cases of reactive lymphoid hyperplasia (RLH) paraffin-embedded tissues. Interphase fluorescence in-situ hybridization (FISH) with dual color breakapart probe was also used to identify rearrangement of bcl-6 gene in 32 cases of nodal DLBCL tissues (including 22 paraffin-embedded samples and 10 fresh samples) and 5 cases of RLH paraffin-embedded tissues.</p><p><b>RESULTS</b>(1) The rates of bcl-6 protein expression in nodal DLBCL, extranodal DLBCL and RLH were 72.7% (16/22), 75.9% (22/29) and 100.0% (10/10) respectively. The rates of CD10 expression were 40.9% (9/22), 41.4% (12/29) and 100.0% (10/10) respectively. All lymphoma samples which expressed CD10 also showed co-expression of bcl-6 protein. (2) The co-expression of bcl-6 and CD10 was observed in 40.9% (9/22) nodal DLBCL and 41.4% (12/29) extranodal DLBCL. Low clinical stage (stage I and II) was more frequently observed in cases with co-expression of bcl-6 and CD10 (P < 0.05). (3) The rates of bcl-6 gene rearrangement in nodal DLBCL was 28.1% (9/32), with 27.3% (6/22) in paraffin-embedded tissues and 30.0% (3/10) in fresh tissues. There was no statistically significant difference found between the two groups (P > 0.05). Bcl-6 gene rearrangement was not found in all the 5 cases of RLH, and there was a significant difference between RLH and DLBCL (P < 0.05).</p><p><b>CONCLUSIONS</b>The rate of bcl-6 protein expression is high in DLBCL cases, and the detection of bcl-6 and CD10 protein co-expression may help in the diagnosis and differential diagnosis of DLBCL. Those DLBCL cases with co-expression of bcl-6 and CD10 may also have a better prognostic implication. On the other hand, bcl-6 gene rearrangement can be identified by interphase FISH with dual color breakapart probe in both paraffin-embedded and fresh lymphoma tissues.</p>

Study of mutations in 5'-noncoding region of BCL-6 gene in B-cell non-Hodgkin's lymphomas / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node.</p><p><b>RESULTS</b>Mutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp.</p><p><b>CONCLUSIONS</b>Mutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.</p>

Mismatch pair defective phenotype in hereditary nonpolyposis colorectal cancer in the Chinese / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To study the protein expression pattern of DNA mismatch repair genes hMSH(2), hMLH(1) and the microsatellite instability (MSI) status in the tumor tissue from hereditary nonpolyposis colorectal cancer in the Chinese.</p><p><b>METHODS</b>Fifty-eight families fulfilling different clinical criteria including Amsterdam Criteria (AC) (22/24 families, 38 tumors), Japanese Criteria (JC) (12/15 families, 16 tumors) and Bethesda Guidelines (BG) (12/19 patients, 13 tumors) were studied. Monoclonal antibodies against hMSH(2), hMLH(1) proteins and a panel of microsatellite markers (5 loci) including BAT26, BAT25, D2S123, D5S346 and D17S250 were used for study.</p><p><b>RESULTS</b>MSI-H was identified in all 22 (100%) AC tumors, with 81.8% (18/22) showing altered hMSH(2) or hMLH(1) expression; in 14/15 (93.8%) JC cancer, 1/1 (100%) JC adenoma, with 45.5% (5/11) showing altered hMSH(2) or hMLH(1) expression; and in 7/13 (53.8%) BG tumors, with 4/7 showing loss of hMSH(2) or hMLH(1) gene expression.</p><p><b>CONCLUSION</b>The frequency of MSI-H and loss of mismatch repair protein are different in the families fulfilling different clinical criteria. Amsterdam Criteria and Japanese Criteria are the two most useful criterion systems for identifying mismatched repair defective tumors. However, Bethesda Guidelines should also be used for detecting more such tumors. The combination of immunohistochemical methods and microsatellite instability analysis is an effective strategy to detect the mismatch repair defective tumors. A close correlation does exist between hMSH(2), hMLH(1) protein expression pattern and MSI status.</p>

Mutation analysis of hMSH2 and hMLH1 genes in Chinese hereditary nonpolyposis colorectal cancer families / 中华病理学杂志

<p><b>OBJECTIVES</b>To determine the germ-line mutations of hMSH2 and hMLH1 genes in Chinese hereditary nonpolyposis colorectal cancer (HNPCC) families' probands or in patients fulfilling different clinical criteria or guidelines; to clarify the nature and distribution of the mutations; to evaluate the sensitivity of different clinical criteria in mutation prediction.</p><p><b>METHODS</b>The entire coding regions (35 exons including exon-intron boundaries) of hMSH2 and hMLH1 genes were directly sequenced in 24 Amsterdam criteria (AC) probands, 15 Japanese criteria (JC) probands (except AC kindreds) and 19 Bethesda guidelines (BG) patients (except two former groups). All available affected and unaffected members from families of those with mutations were screened for mutation.</p><p><b>RESULTS</b>In 16 unrelated families selected by the different clinical criteria, 17 germ-line mutations were found with 11 (64.7%) of hMLH1 and 6 (35.3%) of hMSH2. Two mutations were identified in one of the families. Among the 17 germ-line mutations, 12 had not been reported previously. A diversified mutation spectrum was found, but 6 hMLH1 mutations were found to be concentrated in the region encompassing exon 14, 15 and 16. There was a wide spectrum of mutation type including frame shift, nonsense, splice site mutation, in frame insertion or deletion and missense mutations. The mutation detection rate of hMSH2 and hMLH1 in the AC group was significantly higher than that in the JC group (12/24 vs. 3/15). On the other hand, a low mutation rate (1/19) was detected in 19 BG patients. The mutation cosegregated with disease. Besides, three different genotypes in tumors from probands of mutation-positive families were found.</p><p><b>CONCLUSIONS</b>hMSH2 and hMLH1 mutations in Chinese HNPCC families show a wide spectrum. It seems that hMLH1 gene is involved more frequently than hMSH2 gene in Chinese HNPCC families. Different clinical criteria predict mutations with different sensitivities. The Amsterdam Criteria are most sensitive, while Japanese Criteria are highly practical and the Bethesda Guidelines are also practical to some extent. Gene mutations cosegregate with the disease phenotype. Carriers with no symptom in HNPCC families are most vulnerable groups, follow-ups are required for this group to get early diagnosis and to prevent the development of CRCs.</p>